Graphene oxide-mediated fluorescence turn-on GO-FAM-FRET aptasensor for detection of sterigmatocystin
Pravin Savata Gade, Rutuja Murlidhar Sonkar, Praveena Bhatt
Abstract
27.9 nM) and flow cytometry. GO was immobilized on a polypropylene surface and functionalized with FAM labelled MSA-C6 to develop a simple one-step fluorescence turn-on aptasensor. The linear detection range of the aptasensor was found to be 80-720 ppb with LOD 23.56 ± 4.93 ppb and LOQ 132.43 ± 3.25 ppb. Insignificant interference of salts and detergents as well as negligible cross-reactivity with other structurally similar mycotoxins were observed. Recovery studies in simulated contaminated samples indicated appreciable recoveries (71-89%) using aptasensing assay. The results of the study indicate the successful development of a simple one-step detection platform for STC, useful for the measurement and monitoring of samples for the presence of STC. It also reports a high-affinity aptamer, which can be exploited in other sensing platforms.