Practical genetic control strategies for industrial bioprocesses
Jonathan C. Moore, Itzel Ramos, Stephen Van Dien
Abstract
Optimization of metabolism to maximize production of bio-based chemicals must consistently balance cellular resources for biocatalyst growth and desired compound synthesis. This mini-review discusses synthetic biology strategies for dynamically controlling expression of genes to enable dual-phase fermentations in which growth and production are separated into dedicated phases. Emphasis is placed on practical examples which can be reliably scaled to commercial production with the current state of technology. Recent case studies are presented, and recommendations are provided for environmental signals and genetic control circuits.
Topics & Concepts
Biochemical engineeringBiotechnologyControl (management)BiologyComputational biologyEngineeringComputer scienceArtificial intelligenceMicrobial Metabolic Engineering and BioproductionViral Infectious Diseases and Gene Expression in Insectsthermodynamics and calorimetric analyses