Michler's ethylketone as a novel negative-ion matrix for the enhancement of lipid MALDI tissue imaging
Yiyang Shi, Hao Hu, Qichen Hao, Ran Wu, Lei Wang, Liang Qin, Wei Gu, Haiqiang Liu, Dongxu Jiang, Liya Hong, Yijun Zhou, Xiangyi Liu, Jinchao Feng, Kun Xue, Xiaodong Wang
Abstract
detection and imaging in tissues by MALDI-MSI. The results show several properties of MEK as a powerful MALDI matrix, including strong UV absorption, µm-sized crystals and uniform matrix-coating, super high vacuum chemical stability, low matrix-related ion interference, super soft ionization, and high lipid ionization efficiency.
Topics & Concepts
Matrix (chemical analysis)Absorption (acoustics)ChemistryIonizationCoatingPhotochemistryIonIn situBenzophenoneMaterials scienceCombinatorial chemistryChromatographyOrganic chemistryComposite materialMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry StudiesAnalytical Chemistry and Chromatography