Plasmonic Metasurfaces Based on Pyramidal Nanoholes for High-Efficiency SERS Biosensing
Giovanna Palermo, Massimo Rippa, Ylli Conti, Ambra Vestri, Riccardo Castagna, Giovanna Fusco, Elisabetta Suffredini, Jun Zhou, Joseph Zyss, Antonio De Luca, Lucia Petti
Abstract
An inverted pyramidal metasurface was designed, fabricated, and studied at the nanoscale level for the development of a label-free pathogen detection on a chip platform that merges nanotechnology and surface-enhanced Raman scattering (SERS). Based on the integration and synergy of these ingredients, a virus immunoassay was proposed as a relevant proof of concept for very sensitive detection of hepatitis A virus, for the first time to our best knowledge, in a very small volume (2 μL), without complex signal amplification, allowing to detect a minimal virus concentration of 13 pg/mL. The proposed work aims to develop a high-flux and high-accuracy surface-enhanced Raman spectroscopy (SERS) nanobiosensor for the detection of pathogens to provide an effective method for early and easy water monitoring, which can be fast and convenient.