Metabolic Modeling and Bidirectional Culturing of Two Gut Microbes Reveal Cross-Feeding Interactions and Protective Effects on Intestinal Cells
Belén Hirmas, Naschla Gasaly, Guillermo Orellana, Marco A. Vega, Pedro A. Saa, Martín Gotteland, Daniel Garrido
Abstract
Microbial interactions represent the inner connections in the gut microbiome. By integrating mathematical modeling tools and microbial bidirectional culturing, we determined how two gut commensals engage in the exchange of cross-feeding metabolites, lactate and succinate, for increased growth in two fibers. These interactions underpinned butyrate production in cocultures, resulting in a significant reduction in cellular inflammation and protection against microbial toxins when applied to cellular models.
Topics & Concepts
BiologyMicrobiologyCell biologyGut microbiota and healthDiet and metabolism studiesMicrobial Metabolic Engineering and Bioproduction