Formation of the B9-domain protein complex MKS1–B9D2–B9D1 is essential as a diffusion barrier for ciliary membrane proteins
Misato Okazaki, Takuya Kobayashi, Shuhei Chiba, Ryota Takei, Luxiaoxue Liang, Kazuhisa Nakayama, Yohei Katoh
Abstract
Meckel syndrome (MKS)1, B9 domain (B9D)1, and B9D2 are soluble transition zone (TZ) proteins and share a B9D. We demonstrate the interaction mode of these B9D proteins to be MKS1-B9D2-B9D1 and their interdependent localization to the TZ. We also show that formation of the B9D protein complex is crucial for creating a diffusion barrier for ciliary membrane proteins.
Topics & Concepts
BiologyTransport proteinCell biologyMembrane proteinBiophysicsProtein domainMembraneBiochemistryGeneGenetic and Kidney Cyst DiseasesHedgehog Signaling Pathway StudiesGenetic Syndromes and Imprinting