Observation of peroxynitrite overproduction in cells during 5-fluorouracil treatment<i>via</i>a ratiometric fluorescent probe
Ji‐Ting Hou, Bingya Wang, Yongru Zhang, Beibei Cui, Xinhua Cao, Man Zhang, Yong Ye, Shan Wang
Abstract
We describe a colorimetric and fluorescent probe 3a to detect cellular peroxynitrite with high selectivity and sensitivity. 3a was successfully applied in the bioimaging of exogenous and endogenous peroxynitrite in living cells. The up-regulation of peroxynitrite in cancer cells and normal cells during 5-fluorouracil treatment was finally monitored.
Topics & Concepts
PeroxynitriteFluorescenceOverproductionChemistryBiophysicsBiochemistrySuperoxideBiologyEnzymeOpticsPhysicsNitric Oxide and Endothelin EffectsMolecular Sensors and Ion DetectionSulfur Compounds in Biology