Enhanced response and selective gold nanoparticles/carbon nanotubes biosensor for the early detection of HER2 biomarker
Yahia F. Makableh, Tamara Athamneh, Manar Ajlouni, Sara Hijazi, Aseel Alnaimi
Abstract
A screen-printed carbon electrode was used to develop a biosensor with an improved response and selectivity for detecting human epidermal growth factor receptor 2 (HER2). To investigate the performance of the biosensor, electrochemical characterizations such as impedance spectroscopy, differential pulse and cyclic voltammetry were used, in addition to Fourier transform infrared spectroscopy and atomic force microscopy. The biosensor responds in a quick manner, taking only 5 min to bind to the target protein. Furthermore, the results show that the nano-biosensor has a detection limit of 4.4 Pg/ml. The findings suggest that the biosensor can be used as a potential analytical tool for HER2 detection, particularly at low concentrations, which can be a tool used in the early detection of breast cancer.