Ultrasensitive detection of flap endonuclease 1 using a chemiluminescence optical fiber biosensor with hybridization chain reaction
Jinlan Yang, Yile Qin, Dingsong Li, Yaxue Jia, Jiahui Fu, Li Yang
Abstract
We reported a novel FEN1 sensing approach by combining the COFS and HCR amplification strategy. The approach is accomplished by capturing the 5′-flap sequences produced by FEN1-specific cleavage to trigger the HCR amplification reaction on COFS.
Topics & Concepts
Chain reactionChemiluminescenceEndonucleaseBiosensorChemistryCleavage (geology)Molecular biologyDNAMaterials scienceBiologyChromatographyBiochemistryPhotochemistryFracture (geology)Composite materialAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionRNA Interference and Gene Delivery