Enzyme-Linked Immunosorbent Assay versus Chemiluminescent Immunoassay: A General Overview
Muddasir Khan, Syed Hussain Shah, Muhammad Salman, Abdullah Abdullah, Fawad Hayat, Sajeela Akbar
Abstract
Enzyme-linked immunosorbent assay (ELISA) technique measures antigens, antibodies, and protein reactions in biological samples by enzymatic reactions. The chemiluminescence immunoassay (CLIA) technique determines sample concentrations based on the intensity of the light emitted by a chemical and biological reaction. This review provides an overview to understand the ELSIA and CLIA methods with their types and comparison. ELISA and CLIA methods were compared based on previous literature studies. In conclusion, CLIA is found highly sensitive, specific, and rapid, as compared to ELISA, but CLIA is an expensive method as compared to ELISA.
Topics & Concepts
ChemiluminescenceImmunoassayChemiluminescent immunoassayChemistryChromatographyEnzymeAntibodyAntigenMolecular biologyImmunologyBiochemistryMedicineBiologyBiosensors and Analytical DetectionAdvanced Biosensing Techniques and ApplicationsAdvanced biosensing and bioanalysis techniques