Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry
Mowei Zhou, Carter Lantz, Kyle A. Brown, Ying Ge, Ljiljana Paša‐Tolić, Joseph A. Loo, Frederik Lermyte
Abstract
, native mass spectrometry. Sequence, post-translational modifications, ligand/metal binding, protein folding, and complex stoichiometry can thus all be probed directly. Here, we review recent developments in this new and exciting field of research. While this work is written primarily from a mass spectrometry perspective, it is targeted to all bioanalytical scientists who are interested in applying these methods to their own biochemistry and chemical biology research.
Topics & Concepts
Mass spectrometryTop-down proteomicsChemistryProtein mass spectrometryOrder (exchange)Tandem mass spectrometryChromatographyEconomicsFinanceMass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and ApplicationsIon-surface interactions and analysis