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Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

Jason J. LeBlanc, Jonathan B. Gubbay, Yan Li, Robert Needle, Sandra Radons Arneson, D Marcino, Hugues Charest, Guillaume Desnoyers, Kerry Dust, Ramzi Fattouh, Richard Garceau, Gregory German, Todd F. Hatchette, Robert Kozak, Mel Krajden, Theodore I. Kuschak, Amanda Lang, Paul N. Levett, Tony Mazzulli, Ryan McDonald, Samira Mubareka, Natalie Prystajecky, Candy Rutherford, Marek Smieja, Yang Yu, George Zahariadis, Nathan Zelyas, Nathalie Bastien

2020Journal of Clinical Virology149 citationsDOIOpen Access PDF

Abstract

With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential. This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV-2 in Canadian hospital and public health laboratories, and some commercially available real-time RT-PCR assays. Overall, analytical sensitivities were equivalent between LDTs and most commercially available methods.

Topics & Concepts

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakPandemicVirologyMedicineInfectious disease (medical specialty)OutbreakPathologyDiseaseSARS-CoV-2 detection and testingSARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiology
Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories | Litcius