Dynamic light scattering (DLS) particle size analysis for biomedical nanotechnology
Vikas Shukla, Karishma Niveria, Poonam Shashidhar, Anita Verma
Abstract
Chapter 16 discusses the dynamic light scattering particle size analysis for biomedical nanotechnology and justifies that the introduction of nanostructures in biomedical application is increasing rapidly. Significant efforts are being made to design nano-systems of desired solubility and suspension in media or electrolyte recommended for targeted biomedical research field. To understand these features perfectly, dynamic light scattering (DLS), i.e., photon correlation spectroscopy study explores diffusion behavior of macro/nano molecules in a solution. This technique is also useful to calculate diffusion coefficient and hydrodynamic size. DLS is the recommended methodology in pharmaceuticals to study homogeneity of proteins, nucleic acids, and complexes of protein–protein or protein–nucleic acid preparations, as well as to study protein–small molecule interactions. The descriptive explanation of DLS is covered in this chapter.