<i>In vitro</i>gastrointestinal digestion of buckwheat (<i>Fagopyrum esculentum</i>Moench) protein: release and structural characteristics of novel bioactive peptides stimulating gut cholecystokinin secretion
Hongdong Song, Qingyu Wang, Zhuwei Shao, Xinyue Wang, Hongwei Cao, Kai Huang, Qiqi Sun, Zhenliang Sun, Xiao Guan
Abstract
. Chromatographic separation was performed to isolate peptide fractions involved in CCK secretion, and five novel CCK-releasing peptides including QFDLDD, PAFKEEHL, SFHFPI, IPPLFP, and RVTVQPDS were successfully identified. A sequence length range of 6-8 and marked hydrophobicity (18-28) were observed among the most CCK-releasing peptides. The present study demonstrated for the first time that BPD could stimulate CCK secretion and clarify the structural characteristics of bioactive peptides having CCK secretagogue activity in BPD.
Topics & Concepts
CholecystokininSecretionSecretagogueFagopyrumIn vivoPeptideIn vitroBiochemistryGastrointestinal hormoneDigestion (alchemy)ChemistryBiologyHormonePeptide hormoneReceptorChromatographyBotanyBiotechnologyProtein Hydrolysis and Bioactive PeptidesMicrobial Metabolites in Food BiotechnologySeed and Plant Biochemistry