Acteoside and ursolic acid synergistically protects H <sub>2</sub> O <sub>2</sub> -induced neurotrosis by regulation of AKT/mTOR signalling: from network pharmacology to experimental validation
Yan-Jie Qu, Min-Rui Ding, Chao Gu, Limin Zhang, Rong-Rong Zhen, Jinfang Chen, Bing Hu, Hong‐Mei An
Abstract
CONTEXT: Ursolic acid (UA) and acteoside (ATS) are important active components that have been used to treat Alzheimer's disease (AD) because of their neuroprotective effects, but the exact mechanism is still unclear. OBJECTIVE: Network pharmacology was used to explore the mechanism of UA + ATS in treating AD, and cell experiments were used to verify the mechanism. MATERIALS AND METHODS: + solvent pre-treatment), UA (5 μM), ATS (40 μM), UA (5 μM) + ATS (40 μM). Then apoptosis, mitochondrial membrane potential, caspase-3 activity, ATG5, Beclin-1 protein expression and Akt, mTOR phosphorylation levels were detected. RESULTS: : 250 μM) nerve damage by enhancing cells viability, combating apoptosis, restoring MMP, reducing the activation of caspase-3, lessening the phosphorylation of Akt and mTOR, and increasing the expression of ATG5 and Beclin-1. CONCLUSIONS: -induced neurotrosis by regulation of AKT/mTOR signalling.