A WRKY transcription factor confers broad-spectrum resistance to biotic stresses and yield stability in rice
Daoming Liu, Jun He, Qi Li, Xiao Zhang, Yongsheng Wang, Quanguang Sun, Wenhui Wang, Menglong Zhang, Yunlong Wang, Haosen Xu, Liang Fang, Ling Jiang, Shijia Liu, Liangming Chen, Yunlu Tian, Xi Liu, Ruyi Wang, Zhengguang Zhang, Mawsheng Chern, Oliver Xiaoou Dong, Haiyang Wang, Yuqiang Liu, Pamela C. Ronald, Jianmin Wan
Abstract
Plants are subject to attack by diverse pests and pathogens. Few genes conferring broad-spectrum resistance to both insects and pathogens have been identified. Because of the growth–defense tradeoff, it is often challenging to balance biotic stress resistance and yield for crops. Here, we report that OsWRKY36 suppresses the resistance to insects and pathogens via transcriptional repression of Phenylalanine Ammonia Lyases ( PALs ), a key enzyme in phenylpropanoid pathway in rice. Knocking out OsWRKY36 causes elevated lignin biosynthesis and increased sclerenchyma thickness of leaf sheath, leading to enhanced resistance to multiple pests and pathogens. Additionally, loss of OsWRKY36 also derepresses the transcription of Ideal Plant Architecture 1 ( IPA1 ) and MONOCULM2 ( MOC2 ), resulting in increased spikelet number per panicle and tiller number. These findings provide mechanistic insights into biotic stress tolerance in rice and offer a promising strategy to breed rice cultivars with broad-spectrum resistance to insects and pathogens while maintaining stable yield.