Stop Waiting for Tomorrow: Disk Diffusion Performed on Early Growth Is an Accurate Method for Antimicrobial Susceptibility Testing with Reduced Turnaround Time
Daniel M. Webber, Meghan A. Wallace, Carey-Ann D. Burnham
Abstract
= 0.99) growth correlated well with results from standard conditions. Based on these results, we performed disk diffusion under optimized conditions (6 h growth), using 100 additional clinical isolates, demonstrating a high level of categorical agreement (917 of 950 measurements [96.5%]; 95% confidence interval [CI], 95.2 to 97.5%), as well as no VMEs or MEs. Using early growth for disk diffusion testing is a simple and accurate method for susceptibility testing that can reduce time to results by as much as 18 h, compared to standard incubation, with no additional supply costs or equipment/instrumentation.
Topics & Concepts
DiffusionTurnaround timeAgar diffusion testConfidence intervalBacterial growthAgarAntimicrobialCategorical variableBiologyMicrobiologyMinimum inhibitory concentrationReproducibilityIncubation periodBiomedical engineeringStandard deviationMathematicsClinical microbiologyAgar plateMaterials scienceInterval (graph theory)Antibacterial agentBacteriaMedicineIncubationStatisticsSurgeryBiological systemNuclear medicineExternal quality assessmentChromatographyAnalytical Chemistry (journal)Sensitivity (control systems)ChemistryBacterial Identification and Susceptibility TestingReproductive tract infections researchAntimicrobial Resistance in Staphylococcus