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Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in <i>Arabidopsis</i>

Masaru Fujimoto, Kazuo Ebine, Kohji Nishimura, Nobuhiro Tsutsumi, Takashi Ueda

2020Proceedings of the National Academy of Sciences32 citationsDOIOpen Access PDF

Abstract

Significance The plasma membrane (PM) acts as the interface between intra- and extracellular environments and is thus important for intercellular communication and extracellular signal perception. The composition and amounts of PM proteins are tightly regulated, by molecular mechanisms that remain largely unknown in plant cells. We identified a pair of ANTH domain-containing proteins functioning as adaptors for the retrieval of VAMP72 members, which are components of the membrane fusion machinery, during clathrin-mediated endocytosis. Our results further indicate that the recycling mechanisms of homologous VAMP7 proteins are different in plants and animals, suggesting a divergence of the endocytosis mechanism between these two kingdoms.

Topics & Concepts

EndocytosisArabidopsisCell biologyExtracellularSNARE complexFERM domainMembrane proteinBiologyChemistryMembraneLipid bilayer fusionBiochemistryIntegral membrane proteinReceptorGeneMutantCellular transport and secretionPhotosynthetic Processes and MechanismsPlant Reproductive Biology
Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in <i>Arabidopsis</i> | Litcius