C <sub>3</sub> Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry
Masaki Saito, Yasuhiro Harigae, Guanjie Li, Teizo Asano, Tomohiro Tanaka, Hiroyuki Suzuki, Mika K. Kaneko, Yukinari Kato
Abstract
The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C 3 Mab-2; rat IgG 2b , kappa) using the Cell-Based Immunization and Screening method and showed that C 3 Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C 3 Mab-2 and its recombinant antibody (recC 3 Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.