Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and<i>Haemophilus parasuis</i>co-infection in the lungs of piglets
Jing Zhang, Jing Wang, Xiong Zhang, Zhao Chun-ping, Sixuan Zhou, Chunlin Du, Ya Tan, Yu Zhang, Kaizhi Shi
Abstract
BACKGROUND: (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. OBJECTIVES: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. METHODS: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. RESULTS: ) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. CONCLUSIONS: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.