Ratiometric near-infrared fluorescent probe for nitroreductase activity enables 3D imaging of hypoxic cells within intact tumor spheroids
Janeala J. Morsby, Zhumin Zhang, Alice Burchett, Meenal Datta, Bradley D. Smith
Abstract
, cells with raised Cy7/Cy5 ratio) as a new way to study tumor spheroids. Beyond preclinical imaging, the near-infrared fluorescent molecular probe has high potential for ratiometric imaging of hypoxic tissue in living subjects.
Topics & Concepts
NitroreductaseCyanineFluorescenceSpheroidChemistryFörster resonance energy transferBiophysicsFluorescence-lifetime imaging microscopyBiochemistryIn vitroBiologyEnzymeOpticsPhysicsNanoplatforms for cancer theranosticsCancer, Hypoxia, and MetabolismPhotodynamic Therapy Research Studies