Biomimetic Laccase-Cu2O@MOF for synergetic degradation and colorimetric detection of phenolic compounds in wastewater
Luohao Yuan, Juan Chai, Shiwei Wang, Tong Li, Xinyuan Yan, Jianguo Wang, Hongfeng Yin
Abstract
The successful combination of chemo- and enzyme catalysis technologies in wastewater treatment has presented great potentialities as well as challenges for decades. Herein, laccase (Lac) was immobilized on the Cu2[email protected] by covalent linkage to obtain a stable composite (Cu2[email protected]) with synergistic catalytic activity. The Cu2[email protected] with peroxidase-like activity was in situ synthesized by microwave-assisted assembly method. Cu2[email protected] exhibited high activity recovery, large immobilization capacity, remarkable tolerance to pH and high temperature conditions. Based on the prepared mimic multi-enzyme system, Cu2[email protected] showed broad application prospects in degradation and detection of phenolic compounds. The synergetic catalytic degradation efficiency reached 82.5% in 2h and the limit of detection (LOD) was 0.29 μM in the range of 0-200 μM of 2,4-dichlorophenol (2,4-DCP). Further investigation found that chemo- and enzyme catalysis jointly accelerate the degradation of 2,4-DCP, and the hydroxyl radical (•OH) mediated radical pathway serve a predominant role. Therefore, the chem-enzyme integrated catalyst presented in this work provides a promising platform for organic pollutants degradation and detection by combining the enzymes and Cu2[email protected]