A BBE-like Oxidase, AsmF, Dictates the Formation of Naphthalenic Hydroxyl Groups in Ansaseomycin Biosynthesis
Shuang He Liu, Yuan Yuan Wei, Yin Nan Xing, Yu Chen, Wen Wang, Kai Biao Wang, Yong Liang, Rui Jiao, Bo Zhang, Hui Ming Ge
Abstract
Ansaseomycins are ansamycin-type natural products produced through expression of the asm gene cluster in a heterologous host. A rare berberine bridge enzyme (BBE) like oxidase, AsmF, is encoded in the asm gene cluster. Deletion of asmF led to the accumulation of a series of structurally diverse compounds, all of which lacked the 23-hydroxyl group in naphthalenic motif. Our work demonstrated that AsmF dictated the formation of the naphthalenic hydroxyl group in ansaseomycin biosynthesis.
Topics & Concepts
ChemistryBiosynthesisGene clusterEnzymeHeterologousStereochemistryOxidase testHeterologous expressionBiochemistryGeneRecombinant DNAMicrobial Natural Products and BiosynthesisChemical synthesis and alkaloidsAlkaloids: synthesis and pharmacology