A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance
Hanna Alalam, Fabrice E. Graf, Martin Palm, Marie Abadikhah, Martin Zackrisson, Jonas Boström, Alfred Fransson, Chris Hadjineophytou, Linnéa Persson, Simon Stenberg, Matilda Mattsson, Payam Ghiaci, Per Sunnerhagen, Jonas Warringer, Anne Farewell
Abstract
populations. We recover all previously known and many novel chromosomal gene mutants that affect conjugation efficiency. The new framework holds great potential for rapid screening of compounds that decrease transmission. Further, the platform can easily be adapted to explore interspecies conjugation, plasmid-borne factors, and experimental evolution and be used for rapid construction of strains.
Topics & Concepts
Antibiotic resistancePlasmidAntibioticsGeneTransmission (telecommunications)MicrobiologyBiologyBacteriaGeneticsComputer scienceTelecommunicationsAntibiotic Resistance in BacteriaPharmaceutical and Antibiotic Environmental ImpactsBacterial Genetics and Biotechnology