Long-term mesoscale imaging of 3D intercellular dynamics across a mammalian organ
Yuanlong Zhang, Mingrui Wang, Qiyu Zhu, Yuduo Guo, Bo Liu, Jiamin Li, Xiao Yao, Chui Kong, Yi Zhang, Yuchao Huang, Hai Qi, Jiamin Wu, Zengcai V. Guo, Qionghai Dai
Abstract
at 20 Hz with low phototoxicity. Through the integration of multiple computational imaging techniques, RUSH3D facilitates a 13-fold improvement in data throughput and an orders-of-magnitude reduction in system size and cost. With these advantages, we observed premovement neural activity and cross-day visual representational drift across the mouse cortex, the formation and progression of multiple germinal centers in mouse inguinal lymph nodes, and heterogeneous immune responses following traumatic brain injury-all at single-cell resolution, opening up a horizon for intravital mesoscale study of large-scale intercellular interactions at the organ level.