Allosteric cooperation in a de novo-designed two-domain protein
Fabio Pirro, Nathan W. Schmidt, James Lincoff, Zachary X. W. Widel, Nicholas F. Polizzi, Lijun Liu, Michael J. Therien, Michael Grabe, Marco Chino, Angela Lombardi, William F. DeGrado
Abstract
Significance A major mechanism of evolution involves fusing genes that encode single-domain proteins to create multidomain structures that achieve new functions. Here, we develop methods to design multidomain proteins entirely from scratch and achieve the premier de novo design of an allosterically regulated phenol oxidase that responds to the binding of a synthetic porphyrin.
Topics & Concepts
Allosteric regulationComputational biologyDomain (mathematical analysis)ChemistryComputer scienceBiologyBiochemistryEnzymeMathematicsMathematical analysisProtein Structure and DynamicsMonoclonal and Polyclonal Antibodies ResearchRNA and protein synthesis mechanisms