Simultaneous Analysis of Glucosinolates and Isothiocyanates by Reversed-Phase Ultra-High-Performance Liquid Chromatography–Electron Spray Ionization–Tandem Mass Spectrometry
Silvia Andini, Carla Araya‐Cloutier, Mark Sanders, Jean‐Paul Vincken
Abstract
extracts spiked with 14 GSLs and 15 ITCs indicated that the method generally had good intraday (≤10% RSD) and interday precisions (≤16% RSD). Recovery of the method was unaffected by the extracts and within 71-110% for GSLs and 66-122% for NAC-ITCs. The method was able to monitor the enzymatic hydrolysis of standard GSLs to ITCs in mixtures. Furthermore, GSLs and ITCs were simultaneously determined in Brassicaceae plant extracts before and after myrosinase treatment. This method can be applied to further investigate the enzymatic conversion of GSLs to ITCs in complex mixtures.
Topics & Concepts
ChromatographyChemistryGlucosinolateTandem mass spectrometryMass spectrometrySinapisDerivatizationMyrosinaseBrassicaceaeSinigrinHigh-performance liquid chromatographyLiquid chromatography–mass spectrometryBrassicaBotanyBiologyGenomics, phytochemicals, and oxidative stressFree Radicals and AntioxidantsMoringa oleifera research and applications