Involvement of ethylene in melatonin‐modified photosynthetic‐N use efficiency and antioxidant activity to improve photosynthesis of salt grown wheat
Sheen Khan, Zebus Sehar, Mehar Fatma, Iqbal R. Mir, Noushina Iqbal, Maryam Abbasi Tarighat, Gholamareza Abdi, Nafees A. Khan
Abstract
Abstract The involvement of melatonin in the regulation of salt stress acclimation has been shown in plants in this present work. We found that the GOAL cultivar of wheat ( Triticum aestivum L.) was the most salt‐tolerant among the investigated cultivars, GOAL, HD‐2967, PBW‐17, PBW‐343, PBW‐550, and WH‐1105 when screened for tolerance to 100 mM NaCl. The application of 100 μM melatonin maximally reduced oxidative stress and improved photosynthesis in the cv. GOAL. Melatonin supplementation reduced salt stress‐induced oxidative stress by upregulating the activity of antioxidant enzymes, such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR), and reduced the glutathione (GSH) production. This resulted in increased membrane stability, photosynthetic‐N use efficiency and photosynthesis in plants. The application of 50 μM of the ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG) in the presence of melatonin and salt stress increased H 2 O 2 content but reduced GR activity and GSH, photosynthesis, and plant dry mass. This signifies that melatonin‐mediated salt stress tolerance was related to ethylene synthesis as it improved antioxidant activity and photosynthesis of plants under salt stress. Thus, the interaction of melatonin and ethylene bears a prominent role in salt stress tolerance in wheat and can be used to develop salt tolerance in other crops.