Single-path single-shot phase-shifting digital holographic microscopy without a laser light source
Tatsuki Tahara, Yuichi Kozawa, Ryutaro Oi
Abstract
We propose single-path single-shot phase-shifting digital holographic microscopy (SSP-DHM) in which the quantitative phase information of an object wave is acquired without a laser light source. Multiple phase-shifted holograms are simultaneously obtained using a linear polarizer, a liquid crystal on a silicon spatial light modulator (LCoS-SLM), and a polarization-imaging camera. Complex amplitude imaging of a USAF1951 test target and phase imaging of transparent HeLa cells are performed to show its quantitative phase-imaging ability. We also conduct an experiment for the motion-picture imaging of transparent particles to highlight the single-shot imaging ability of SSP-DHM.
Topics & Concepts
OpticsHolographyDigital holographic microscopyDigital holographyMaterials sciencePhase imagingMicroscopySpatial light modulatorPhase modulationLaserLaser lightPhase (matter)Spatial frequencyDigital micromirror deviceComputer-generated holographyPhase-contrast imagingSiliconHolographic displayLight sheet fluorescence microscopyOptoelectronicsLight intensityDigital imagingPhase retrievalMicroscopeImage processingPtychographyWavefrontLiquid crystalHolographic interferometryAmplitudeLaser beamsLight scatteringOptical microscopeCoherent diffraction imagingFourier opticsUltrashort pulseDigital Holography and MicroscopyPhotorefractive and Nonlinear OpticsRandom lasers and scattering media