Induction of Protective Antiplague Immune Responses by Self-Adjuvanting Bionanoparticles Derived from Engineered Yersinia pestis
Xiuran Wang, Amit K. Singh, Xiangmin Zhang, Wei Sun
Abstract
A Yersinia pestis mutant synthesizing an adjuvant form of lipid A (monophosphoryl lipid A, MPLA) displayed increased biogenesis of bacterial outer membrane vesicles (OMVs). To enhance the immunogenicity of the OMVs, we constructed an Asd-based balanced-lethal host-vector system that oversynthesized the LcrV antigen of Y. pestis , raised the amounts of LcrV enclosed in OMVs by the type II secretion system, and eliminated harmful factors like plasminogen activator (Pla) and murine toxin from the OMVs.
Topics & Concepts
Yersinia pestisImmunogenicityBiologyMicrobiologyYersiniaBacterial outer membraneLipid ASecretionImmune systemAdjuvantAntigenVirologyLipopolysaccharideVirulenceBacteriaImmunologyEscherichia coliBiochemistryGeneGeneticsYersinia bacterium, plague, ectoparasites researchVector-borne infectious diseasesVibrio bacteria research studies