Immobilization of Recombinant Fluorescent Biosensors Permits Imaging of Extracellular Ion Signals
Sandra Burgstaller, Helmut Bischof, Thomas Rauter, Tony Schmidt, Rainer Schindl, Silke Patz, Bernhard Groschup, Severin Filser, Lucas van den Boom, Philipp Sasse, Robert Łukowski, Nikolaus Plesnila, Wolfgang F. Graier, Roland Malli
Abstract
measurements on cell surfaces. Our results suggest that the approach can be greatly expanded by immobilizing various biosensors on extracellular surfaces to quantitatively visualize microenvironmental transport and signaling processes in different cell culture models and other experimental settings.
Topics & Concepts
BiosensorExtracellularBiotinylationBiophysicsFluorescenceIntracellularLive cell imagingChemistryRecombinant DNAFörster resonance energy transferCellBiochemistryNanotechnologyCell biologyBiologyMaterials scienceGenePhysicsQuantum mechanicsIon channel regulation and functionNeuroscience and Neuropharmacology ResearchReceptor Mechanisms and Signaling