High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
Cristina I. Amador, Rune Overlund Stannius, Henriette Lyng Røder, Mette Burmølle
Abstract
The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.
Topics & Concepts
BiofilmCrystal violetFluorescenceThroughputHigh-throughput screeningPlate readerFluorescence-lifetime imaging microscopyChemistryBiophysicsBiological systemBiologyMicrobiologyBiochemistryBacteriaOpticsComputer sciencePhysicsGeneticsWirelessTelecommunicationsBacterial biofilms and quorum sensingMicrobial Community Ecology and PhysiologyMarine Biology and Environmental Chemistry