Tryptophan-Based Hyperproduction of Bioindigo by Combinatorial Overexpression of Two Different Tryptophan Transporters
Hyun‐Jin Kim, Sion Ham, Nara Shin, Jeong Hyeon Hwang, Suk Jin Oh, Tae‐Rim Choi, Jeong Chan Joo, Shashi Kant Bhatia, Yung‐Hun Yang
Abstract
produced 8.77 mM (2.3 g/l) of bioindigo after 66 h of culture. The produced bioindigo was further recovered using a simple method and used as a watercolor dye, showing good mixing with other colors and color retention for a relatively long time. This study presents an effective strategy for enhancing indigo production using a combination of transporters.
Topics & Concepts
TryptophanChemistryTransporterBiochemistryComputational biologyBiologyGeneAmino acidPharmacological Effects of Natural CompoundsGinseng Biological Effects and ApplicationsPlant biochemistry and biosynthesis