A novel Fe3O4/CuOx nanozyme: Intrinsic peroxidase-like activity to kill bacteria and sterilize wounds
Mengyu Ding, Liqiang Du, Aiying Guo, Baoping Qin, Xinyue Zhang, Kunze Wu, Xiuping Wang, Aijun Cai
Abstract
Herein, we report a Fe 3 O 4 /CuO x composite with intrinsic highly peroxidase-like activity. The Fe 3 O 4 /CuO x composite maintained high activity in the normal skin pH range (4.0–6.0) and over a wide temperature range (25–60 °C). The prepared Fe 3 O 4 /CuO x exhibited good bactericidal properties against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) at low H 2 O 2 concentration (100 μM). The composite could also effectively destroy the biological film and prevent the emergence of new bacterial film. In addition, Fe 3 O 4 /CuO x was easily separated from the media by external magnets and reused five times while maintaining high antibacterial activity. The predominant free radicals (•OH and 1 O 2 ) were identified during the sterilization process using a trapping experiment and electron spin resonance (ESR). In addition, the dressing of Fe 3 O 4 /CuO x was designed to accelerate skin wound healing, maintaining good cytocompatibility significantly. This composite Fe 3 O 4 /CuO x is promising for a wide range of uses in bacterial management.