Immunostimulatory Effect of Heat-Killed Probiotics on RAW264.7 Macrophages
Hye-Ji Noh, Jung Min Park, Yoo Jin Kwon, Kyunghwan Kim, Sung Yurb Park, Insu Kim, Jong Hyun Lim, Byoung Kook Kim, Byung‐Yong Kim
Abstract
SRK414 (LF4), via phagocytosis, nitric oxide (NO), and pro-inflammatory cytokine production in macrophages. We thus found that heat-killed LP8 could promote the clearance of foreign pathogens by enhancing the phagocytosis of macrophages. Treatment with heat-killed LP8 induced the production of NO and pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β. In addition, heat-killed LP8 suppressed the production of NO and cytokines in LPS-induced RAW264.7 cells, suggesting that heat-killed LP8 exerts immunomodulatory effects depending on the host condition. In sum, these results indicate that heat-killed LP8 possesses the potential for immune modulation while providing a molecular basis for the development of functional probiotics prepared from inactivated bacterial cells.