Analyzing Criteria Affecting the Functionality of G-Quadruplex-Based DNA Aptazymes as Colorimetric Biosensors and Development of Quinine-Binding Aptazymes
Yasaman Ahmadi, Regina Soldo, Krista Rathammer, Laura Eibler, Ivan Barišić
Abstract
, and ATP for this detection concept. As a result, six novel aptazymes were developed for the specific detection of quinine based on two quinine-binding aptamers. The rest of designed probes, however, hardly showed significant functionality. To uncover the reasons, we performed enzyme-linked oligonucleotide assays to find how the affinity of aptamers is affected once conjugated to the DNAzyme sequence or upon integration into the aptazyme probe. Furthermore, we investigated the impact of the structure-switching functionality in the parent aptamer and the effect of the reaction matrix on the efficiency of probes.
Topics & Concepts
AptamerDeoxyribozymeChemistryBiosensorG-quadruplexHeminOligonucleotideDNACombinatorial chemistryBiochemistryNanotechnologyComputational biologyMolecular biologyEnzymeBiologyMaterials scienceHemeAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryBacteriophages and microbial interactions