Preliminary Phytochemical Screening and in Vitro Anthelmintic Effects of Acmella Paniculata Plant Extracts
Rajeshwar Yerra, R Lalitha
Abstract
<strong>ABSTRACT</strong> Development of resistant to most of commercially available anthelmintic became a sever problem worldwide. Pet ether, chloroform, ethyl acetate and methanol extracts of <em>Acmella paniculata </em>were evaluated for there anthelmintic activity using <em>Pheretima posthuma</em> model (Indian earthworm). The preliminary phytochemical analysis showed the presence of alkaloids, saponins, flavonoids and tannins in whole plant. Three concentrations (0.075, 0.15 and 0.22w/v) of each extracts were used for this study involve the determination of time of paralysis and time of death of the worm. Extracts obtained from whole plant not only paralyzed but also killed the earth worms. It was observed that both ethyl acetate, pet ether, chloroform and methanol extracts showed a remarkable Anthelmintic potential against intestinal parasitism. Further, there is scope to evaluate those active principles of <em>Acmella paniculata</em> whole plant for their Anthelmintic activity to open the new door for natural Anthelmintic. <strong>References</strong> Ajaiyeoba EO, Onocha PA, Olarenwaju OT, (2001). <em>In-vitro</em> anthelmintic properties of <em>Buchholzia coriaceae</em> and Gynandropsis gynandro extracts. <em>Pharm. Biol,</em> 217-220. Bundy DA, (2000). Immuno epidemiology of intestinal helminthic infection: <em>The global burden of intestinal nematode disease.</em> Chatterjee KD. (1967). Parasitology, Protozoology and Helminthalogy .6<sup>th</sup> edition, Calcutta: in <em>Guha ray Sree Saraswaty press</em> <em>LTD</em>. Dash G.K., Suresh P., Kar D.M, Ganraty S., Panda S.D, (2002). Evaluation of Evolvulvs alsinoids linn. For anthelmintic and antimicrobial activities .<em>J Nat Rem</em>, 82-185. Extraction Technologies for Medicinal and Aromatic plants. Scientific editors: Sukhdev Swami Handa, <em>Suman preet singh</em>. 21-24. Grime AS, Bhalke RD, Ghogare PB, Tambe VD, Jadhav RS and Nirmal SA, 2006). Comparative in vitro anthelmintic activity of <em>Menthe piperita</em> and <em>Lantana camara</em> from western India. Dhaka University .<em>Jour. Pharm. Sci</em>. 5-7. John <em>J</em>, Mehta A, Shukal S, Mhta P<em>,</em> (2001). Anthelmintic activity of Cassiatora leaves. Pharm. Biol. Volume 39, 217-220. Kuo-Fang Chung, Yoshiko Kono Chiu-Mei Wang, and Ching. (2008). Botanical Studies Note on <em>Acmella (Asteraceae</em>) in Taiwan. Volum-49. 73-82. Lingaiah M And Nagaraja Rao P. (2013). An Ethnobotanical Survey Of Medicinal Plants Used By Traditional Healers Of Adilabad District, Andhra Pradesh, India. Biolife.1(1):17-23, 2013 Nirmal SA, Nikalje AG, Jadhav RS, Tambe VD. (2001). Anthelmintic activity of <em>Martynia annua</em> roots. <em>Indian Drugs.</em> 44:772–773.