Stability matters: evaluating the long-term performance of AuNP–DNA conjugates in lateral flow assays through varied conjugation methods and storage buffers
Celia Fuentes‐Chust, Claudio Parolo, Andrew Piper, Arben Merkoçi
Abstract
conjugates demonstrated initial functionality but degraded due to butanol residues and aggregation. A complementary analysis using electrophoretic mobility shift assays (EMSAs), UV-Vis spectroscopy, and dynamic light scattering (DLS) provided essential insights into conjugate behavior. These results emphasize the importance of buffer selection and conjugation techniques for optimizing DNA-AuNP conjugates in LFAs, ensuring their reliability and effectiveness in diagnostic applications.
Topics & Concepts
ConjugateTerm (time)Stability (learning theory)DNAFlow (mathematics)Materials scienceCombinatorial chemistryNanotechnologyBiological systemChemistryBiophysicsComputer scienceBiologyBiochemistryMathematicsMechanicsPhysicsMachine learningMathematical analysisQuantum mechanicsAdvanced biosensing and bioanalysis techniquesMolecular Biology Techniques and ApplicationsCRISPR and Genetic Engineering