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First report of Myroides phaeus bacteraemia identified by Polymerase chain reaction and genetic sequencing

Giancarlo Pérez‐Lazo, Adriana Morales-Moreno, Fernando Soto‐Febres, H. Jove-Químper, Liliana Morales-Castillo, Carlos Palomares-Reyes, Juana del Valle-Mendoza, Miguel Ángel Aguilar-Luis, Wilmer Silva-Caso

2020IDCases18 citationsDOIOpen Access PDF

Abstract

We report the first case of Myroides phaeus isolated from blood, causing bacteremia in an immunocompromised patient using the automated MicroScan Walk Away 96 system, followed by bacterial identification by amplification-sequencing of the 16S rDNA. The sequences obtained were compared with the reference sequence of the BLAST ® platform - National Library of Medicine, USA, and the isolation was identified as Myroides phaeus strain with 99.67 % identity in Blast report. In the literature we did not find previous reported cases of infections by this bacterium, however its pathogenic role is still controversial; therefore, this isolation alerts us to carry out an exhaustive surveillance of other possible acquisition routes.

Topics & Concepts

Isolation (microbiology)BacteremiaPolymerase chain reactionMedicineMicrobiologyDNA sequencing16S ribosomal RNAComputational biologyGeneticsBacteriaDNABiologyGeneAntibioticsInfections and bacterial resistanceBacterial biofilms and quorum sensingPlant Pathogenic Bacteria Studies
First report of Myroides phaeus bacteraemia identified by Polymerase chain reaction and genetic sequencing | Litcius