Clinical Utility of Universal Broad-Range Polymerase Chain Reaction Amplicon Sequencing for Pathogen Identification: A Retrospective Cohort Study
Andrew D. Kerkhoff, Rachel L. Rutishauser, Steve Miller, Jennifer M. Babik
Abstract
We assessed the real-world utility of universal broad-range polymerase chain reaction sequencing for pathogen detection. Among 1062 clinical samples, 107/1062 (10.1%) had a clinically significant, positive result, with substantial variation by specimen type. Clinical management was changed in 44/1062 (4.1%). These data can help maximize utility of this emerging diagnostic.
Topics & Concepts
AmpliconPolymerase chain reactionPathogenIdentification (biology)Computational biologyMolecular diagnosticsCohortBiologyRetrospective cohort studyMedicineBioinformaticsGeneticsInternal medicineGeneEcologyBacterial Identification and Susceptibility TestingInfective Endocarditis Diagnosis and ManagementMolecular Biology Techniques and Applications