Amplification-free miRNA detection with CRISPR/Cas12a system based on fragment complementary activation strategy
Shuang Zhao, Qiuting Zhang, Ran Luo, Jiudi Sun, Cheng Zhu, Dianming Zhou, Xiaoqun Gong
Abstract
imaging, effectively recognizing tumor cells. The FCAS not only broadens the scope of CRISPR/Cas12a system target identification but also unlocks the potential for in-depth studies of CRISPR technology in many diagnostic settings.
Topics & Concepts
CRISPRFragment (logic)Computational biologymicroRNABiologyChemistryComputer scienceGeneticsGeneAlgorithmCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery