Systems analysis of plasma IgG intact <i>N</i>-glycopeptides from patients with chronic kidney diseases <i>via</i> EThcD-sceHCD-MS/MS
Yong Zhang, Shanshan Zheng, Yonghong Mao, Wei Cao, Lijun Zhao, Changwei W. Wu, Jingqiu Cheng, Fang Liu, Guisen Li, Hao Yang
Abstract
In this study, we established a sample preparation procedure for the separation and purification of IgG and a rapid detection procedure for the IgG subclasses with intact N -glycopeptides using EThcD-sceHCD-MS/MS for the first time.
Topics & Concepts
GlycopeptideGlycosylationChemistryKidney diseaseTrypsinAntibodyImmunoglobulin GImmunologyBiochemistryInternal medicineMedicineEnzymeAntibioticsGlycosylation and Glycoproteins ResearchMonoclonal and Polyclonal Antibodies ResearchBlood groups and transfusion