Immunogenic Potency of Formalin and Heat Inactivated <i>E. coli</i> O157:H7 in Mouse Model Administered by Different Routes.
Nasim Arshadi, Seyed Latif Mousavi Gargari, Jafar Amani, Shahram Nazarian
Abstract
BACKGROUND: O157:H7 and developing a specific immunity in animals would be invaluable. The administration of Whole Cell Vaccines (WCV) is a well-established method of vaccination against bacterial infections. Route of administration, inactivation and using suitable adjuvant have significant effects on the characteristics and efficacy of WCV. METHODS: O157:H7 and feces were monitored for shedding. RESULTS: O157:H7 induced significant specific levels of IgG antibodies but did not lead to significant antigen-specific IgA rise in feces, whereas oral immunization elicited significant levels of IgG antibodies with some animals developing antigen-specific IgA in feces. CONCLUSION: O157:H7 is highly immunogenic and can induce protective immune responses via oral immunization.