Rewiring the “Push-Pull” Catalytic Machinery of a Heme Enzyme Using an Expanded Genetic Code
Mary Ortmayer, Karl Fisher, Jaswir Basran, Emmanuel Wolde-Michael, Derren J. Heyes, Colin Levy, Sarah L. Lovelock, J. L. Ross Anderson, Emma Lloyd Raven, Sam Hay, Stephen E. J. Rigby, Anthony P. Green
Abstract
P, suggesting that a common mechanistic strategy is employed by enzymes using distinct electron transfer pathways. Our study highlights how noncanonical active site substitutions can be used to directly probe and deconstruct highly evolved bioinorganic mechanisms.
Topics & Concepts
ChemistryHemeLigand (biochemistry)HistidineElectron transferReactivity (psychology)PeroxidaseStereochemistryMutagenesisCatalysisElectron transport chainEnzymeCombinatorial chemistryPhotochemistryMutationBiochemistryReceptorPathologyGeneMedicineAlternative medicinePhotosynthetic Processes and MechanismsCRISPR and Genetic EngineeringMetal-Catalyzed Oxygenation Mechanisms