Revealing the signaling regulation of hydrogen peroxide to cell pyroptosis using a ratiometric fluorescent probe in living cells
Guanqing Yang, Tong Zhu, Dong Wang, Zhengjie Liu, Ruilong Zhang, Guangmei Han, Xiaohe Tian, Bianhua Liu, Ming‐Yong Han, Zhongping Zhang
Abstract
A ratiometric fluorescent probe with a large emission shift was developed for the accurate measurement of hydrogen peroxide (H2O2) in sophisticated pyroptosis signaling pathways. The results reported here demonstrate that H2O2, as a principal member of ROS, is a critical upstream signaling molecule in regulating pyroptosis.
Topics & Concepts
PyroptosisHydrogen peroxideFluorescenceLiving cellChemistryNanotechnologyBiophysicsMaterials sciencePhotochemistryCell biologyProgrammed cell deathApoptosisBiochemistryOpticsBiologyPhysicsSulfur Compounds in BiologyMolecular Sensors and Ion DetectionNitric Oxide and Endothelin Effects