Water-soluble fluorescent probes for differentiating cancer cells and normal cells by tracking lysosomal viscosity
M. Liu, Jintao Weng, Shumei Huang, Wenjin Yin, Huatang Zhang, Yin Jiang, Yang Liu, Hongyan Sun
Abstract
Lysosomal viscosity is a significant parameter of lysosomes and closely related to various diseases. Herein, two fluorescent probes, Lyso-vis-A and Lyso-vis-B, were developed, which demonstrate diverse advantages, including great water solubility, lysosome targeting ability and viscosity sensitivity. In particular, Lyso-vis-A exclusively showed fluorescence response toward viscosity but was not influenced by pH changes, rendering it a selective lysosomal viscosity probe. Furthermore, Lyso-vis-A was successfully applied to monitor lysosomal viscosity variations in living cells and differentiate cancer cells and normal cells.
Topics & Concepts
FluorescenceLysosomeViscosityChemistryBiophysicsBiochemistryBiologyEnzymeMaterials scienceQuantum mechanicsComposite materialPhysicsDendrimers and Hyperbranched PolymersMolecular Sensors and Ion DetectionPorphyrin and Phthalocyanine Chemistry