Enzymatic Photodecarboxylation on Secondary and Tertiary Carboxylic Acids
Jie Zheng, Zhuanglin Shen, Jin‐Ming Gao, Jiahai Zhou, Yang Gu
Abstract
Photoenzymatic decarboxylation of bulky secondary and tertiary carboxylic acids catalyzed by engineered Chlorella variabilis fatty acid photodecarboxylase ( Cv FAP) is reported. Rational design and directed evolution of wild-type Cv FAP are used to improve the reactivity and expand potential applications. Moreover, engineered Cv FAP can catalyze light-driven kinetic resolution of α-substituted carboxylic acid. Our work sheds light on the production of chiral building blocks and bioactive molecules from bulky carboxylic acids via the photoenzymatic way.
Topics & Concepts
ChemistryDecarboxylationCarboxylic acidKinetic resolutionReactivity (psychology)MoleculeEnzymeRational designOrganic chemistryCatalysisCombinatorial chemistryEnantioselective synthesisNanotechnologyPathologyMaterials scienceMedicineAlternative medicineEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionAlgal biology and biofuel production