CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern
Yuanhao Liang, Hongqing Lin, Lirong Zou, Jianhui Zhao, Baisheng Li, Haiying Wang, Jing Lu, Jiufeng Sun, Xingfen Yang, Xiaoling Deng, Shixing Tang
Abstract
We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to be quickly developed, continuously updated, and easily implemented for screening of SARS-CoV-2 variants in resource-limited settings. This approach can be adapted for emerging mutations and implemented in laboratories already conducting SARS-CoV-2 nucleic acid amplification tests using existing resources and extracted nucleic acid.
Topics & Concepts
BiologyConcordanceGeneticsVirologyPrimer (cosmetics)Computational biologyMutationOpen reading frameVirulenceSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)GenePandemicPolymerase chain reactionVirusCoronavirusCoronavirus disease 2019 (COVID-19)Sequence analysisMutation testingMutation rateDNA sequencingCRISPR and Genetic EngineeringSARS-CoV-2 and COVID-19 ResearchCOVID-19 Clinical Research Studies