Investigation of wound healing and anti-inflammatory activity of Senna occidentalis leaf extract, and in silico screening for both activities
Md. Abu Shyeed, Mahci Al Bashera, Ovijit Sarkar Sazal, Md.Moktar Ali, MM Hossain, Henry Sandip Kumar Mondol, Mohammad Ali Chowdhury, Khan Rajib Hossain, Md. Tamzid Hossain Molla
Abstract
Senna occidentalis, synonym Cassia occidentalis, is a native American pantropical plant species previously classified under the genus Cassia. This study is for testing and to discover new potent phytochemicals from this plant as wound healing and anti-inflammatory agents. The Excision and Assay of Red Blood Cell (RBC) Membrane Stabilization for Anti-Inflammatory Activity Test Method was used to test how well extracts of S. occidentalis leaves from methanol, n-hexane, chloroform, and absolute alcohol helped wounds heal and stopped inflammation. In silico is another method for finding potent phytochemicals for both activities. These leaf extracts effectively cure wound areas and promote re-epithelialization. The methanol extract exhibited maximum wound healing (95.04 %) and anti-inflammation (62.94 %) activity compared to their other extracts, standard, and control groups. In silico molecular docking of Apigenin, Aloe-emodin with GSK-3B protein, and 1-Methoxynaphthalene, Quinine with COX-2 protein showed binding affinity in (kj/mol) of −8.4, −8.6, and −7.3, −7.7, for wound healing and anti-inflammatory activity, respectively, in their binding sites with stability. They support the "Lipinski Rule of Five." This plant leaf extract is recommended as a traditional medicine and an alternative, complementary treatment for its continued contribution to drug discovery and development.