Transition of antimicrobial resistome in wastewater treatment plants: impact of process configuration, geographical location and season
Ryo Honda, Norihisa Matsuura, Sovannlaksmy Sorn, Sawako Asakura, Yuta Morinaga, Than Van Huy, Muhammad Adnan Sabar, Yalkhin Masakke, Hiroe Hara-Yamamura, Toru Watanabe
Abstract
Abstract Antimicrobial resistome in wastewater treatment plants was investigated via shotgun metagenomic analysis over a variety of geographical locations, seasons, and biological treatment configurations. The results revealed that the transition of the antimicrobial resistome occurred at two locations during wastewater treatment, which resulted in a distinctive antimicrobial resistome in influent wastewater, activated sludge, and treated effluent. The antimicrobial resistome in influent wastewater was characterized by a high abundance of antibiotic resistance genes (ARGs) on clinically important drugs, whereas sludge retained a higher abundance of multidrug ARGs associated with efflux pump. Seasonality was the primary factor affecting antimicrobial resistome in influent wastewater, which partially succeeded to the subsequent resistome of activated sludge and treated effluent. Importantly, some ARGs on clinically important drugs in influent wastewater passed through the biological treatment to be discharged in the treated effluent, except in the membrane bioreactor process.